If incorrect amt was entered on line 221 i have unchecked investment box under income expse amt still remains in deduction summary as carrying charges, how to remove it?

You'll need to remove the entry on the investment expenses screen itself. Just removing the checkbox won't erase an entry.

To return to the investment expenses screen, use the Find button to search/select the term Investment Expenses. Once you're on that screen, click continue to proceed to the entries that need to be made zero.