Yes, you can.
This FAQ tells you how to submit your tax return to CRA using the NETFILE service in TurboTax Online: https://turbotax.community.intuit.ca/community/federal-taxes-netfile/help/in-the-online-edition-of-turbotax-how-do-i-submit-my-tax-return-to-cra-using-the-netfile-service/00/873715
This FAQ tells you how to submit your tax return to CRA using the NETFILE service in TurboTax CD/Download: https://turbotax.community.intuit.ca/community/federal-taxes-netfile/help/in-the-cd-download-edition-of-turbotax-how-do-i-submit-my-tax-return-to-cra-using-the-netfile/00/873419