Yes, you can prepare your return separately from your spouse. When you start your TurboTax Online return, when it asks "Who are you preparing this return for?" answer "Myself". You will still have to provide some basic info on your spouse, as well as their income. Please note that if you use the same account you both will be able to see each others return.
If you do not want to see each others returns, you will need to create a new TurboTax Online account.
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