Yes, ON428 line 6097 can apply to a spouse.
From the guide:
"You can include a spouse or common-law partner with a mental or physical impairment if you are claiming a disability
amount transferred from your spouse or common-law partner on line 3 of your Schedule ON(S2), Provincial Amounts
Transferred From Your Spouse or Common-Law Partner."
If the CRA has approved a T2201 application for the Disability Tax Credit for your spouse, make sure to indicate this in TurboTax by going to Deductions > Disability Amount.
