No. Your wife's return will be a separate return, even if you choose to prepare both your returns at the same time. It will appear as one file, but two returns will be sent to the Canada Revenue Agency’s (CRA) and you will be charged for 2 returns,

If your business is not incorporated, then you can use TurboTax Self-Employed and include your income & expenses on your return. But if your business is incorporated, you'll need to file a separate T2 return for it, using TurboTax Business Inc.